Following with 48 h sulbactam incubation or not,the culture was under 2 h oxygen glucose deprivation(OGD),and then the medium was replaced by the ba-
The mortality rate of gastric cancer worldwide is as high as 70%, despite the development of novel therapeutic strategies. One reason for the high mortality is the rapid and uninhibited spread of the disease, such that the majority of patients are diagnosed at a stage when efficient therapeutic treatment is not available. Therefore, in-depth research is needed to investigate

the mechanism of gastric cancer metastasis and invasion to improve outcomes and provide biomarkers for early diagnosis. The mitogen-activated protein kinase(MAPK) signaling pathway is widely expressed in multicellular organisms, LY294002 with critical roles in multiple biological processes, such as cell proliferation, death, differentiation, migration, and invasion. The MAPK PLX4032半抑制浓度 pathway typically responds to extracellular stimulation. However, the MAPK pathway is often involved in the occurrence and progression of cancer when abnormally regulated. Many studies have researched the relationship between the MAPK signaling pathway and cancer metastasis and invasion, but little is known about the important roles that the MAPK signaling pathway plays in gastric cancer. Based on an analysis of published data, this review aims to summarize the important role that the MAP

kinases play in the invasion and metastasis of gastric cancer and attempts to provide potential directions for further research and clinical treatment.
背景:细胞外基质磷酸化糖蛋白基因在骨的矿化和吸收、成骨细胞与破骨细胞的平衡中起重要的作用,研究细胞外基质磷酸化糖蛋白的功能及其调控机制可为骨质疏松症的治疗提供新的思路。目的:分析转录因子Runx2在小鼠前成骨细胞中对细胞外基质磷酸化糖蛋白基因启动子的调控作用,进而初步研究转录因子Runx2在骨形成发育过程中的作用。方法:首先根据Genbank中Runx2的基因序列构建Runx2真核表达载体;然后利用双荧光素酶基因检测报告系统分析Runx2对不同长度的细胞外基质磷酸化糖蛋白基因启动子转录活性的影响,以确定Runx2有明显作用的启动子区段,分析3种MAPK信号通路抑制剂调控Runx2对细胞外基质磷酸化糖蛋白基因启动子转录活性的影响;最后利用定时定量RT-PCR法分析Runx2对细胞外基质磷酸化糖蛋白基因启动子表达活性的影响。结果与结论:成功构建Runx2真核表达载体;双荧光素酶基因检测报告系统分析显示Runx2能够上调细胞外基质磷酸化糖蛋白基因启动子在前成骨细胞中的转录活性,在(-300-+66)366

以及 bp片段区域内上调效果较为显著,Runx2可通过激活MEK激酶MAPK通路上调细胞外基质磷酸化糖蛋白启动子活性;定时定量RT-PCR法检测再次验证Runx2上调细胞外基质磷酸化糖蛋白基因启动子的表达水平。提示转录因子Runx2可以通过MEK激酶MAPK信号通路对细胞外基质磷酸化糖蛋白基因表达进行调节,为探讨细胞外基质磷酸化糖蛋白在骨形成发育过程中的意义奠定基础。
目的观察鞘内注射重组大鼠脂质运载蛋白-2(LCN2)对大鼠吗啡镇痛效能的影响,并探讨其分子机制。方法健康雄性SD大鼠32只,体重150~180g,采用随机数字表法将鞘内置管成功的大鼠随机分为四组(n=8):Ⅰ组为对照组:鞘内注射MES缓冲液10μl,Ⅱ组、Ⅲ组和Ⅳ组分别鞘内注射10μl含LCN2蛋白0.02、0.2、2μg的MES溶液,每天1次,连续5d。分别在鞘内给药前和给药后第6、7、8天皮下注射吗啡10mg/kg,吗啡注射前和注射后45min测定大鼠热辐射缩足潜伏期(PWTL),并计算最大可能镇痛效应百分比(MPE)。第8天行为学测试结束后处死动物,取脊髓腰膨大,采用Western blot法检测磷酸化p38丝裂原活化蛋白激酶(p-p38 MAPK)的表达;采用免疫组织化学法检测星型胶质细胞标志物胶质纤维酸性蛋白(GFAP)的表达。结果与Ⅰ组和给药前比较,Ⅱ组大鼠鞘内给药后基础PWTL和MPE差异无统计学意义,Ⅲ组、Ⅳ组鞘内给药后第6、7、8天基础PWTL和MPE均明显降低(P
目的:探讨二十二碳六烯酸(DHA)对卵巢癌耐药细胞的耐药逆转作用及其机制。方法:利用MTT评估细胞对紫杉醇耐药性的改变,罗单明实验验证细胞的药物外排能力,流式细胞仪检测细胞周期的分布,实时定量PCR及蛋白质印迹检测多药耐药蛋白1(MDR1)的mRNA水平、耐药相关蛋白及通路蛋白的蛋白水平。结果:DHA作用48 h后A2780/T对紫杉醇的半数抑制率(IC50)下降(P<0.